Coding

Part:BBa_M11062:Design

Designed by: Dan Nelson   Group: UtahState BE5930 - S09 UtahState BE5930 - S10   (2009-04-13)


pfaC gene for omega-3 polyunsaturated fatty acid synthase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 5147
    Illegal BglII site found at 5787
    Illegal BamHI site found at 4218
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 408
    Illegal NgoMIV site found at 958
    Illegal NgoMIV site found at 1459
    Illegal NgoMIV site found at 1587
    Illegal NgoMIV site found at 2377
    Illegal NgoMIV site found at 3411
    Illegal AgeI site found at 520
    Illegal AgeI site found at 3027
    Illegal AgeI site found at 4912
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Mutations were made to the orginal sequence to exclude Pst1 sites as well as Xba1.


Source

Photobacterium profundum SS9 chromosome 1: NC_006370 (JGI)

References